Dwi Murti Puspitaningtyas
Elizabeth Handini


Coelogyne is a native orchid to Indonesia which has a relatively large flower and attractive colors. Coelogyne seed storage needs to be done to conserve the diversity of the species. The experiment of  orchid seeds  storage on seven species of Coelogyne at temperature -20°C have been carried out for four years at Center for Plant Conservation Botanic Gardens–Indonesian Institute of Sciences. The experimental design used completely randomized design, with media treatment as factors for each species of Coelogyne. Four kinds of media for seed germination test were KCA (Knudson C), KC (modified Knudson C), VW (modified Vacin and Went), and HS (modified Hyponex). Seed viability test was observed after the seeds were stored for 0, 1, 2, 3, 6, 9, 12, 18, 24, 36, 48 months. The results showed that the seed of Coelogyne able to germinate in four experimental media. Coelogyne seed viability declined in three months, six months, nine months, and so on depending on the Coelogyne species. Seeds of Coelogyne which can be stored for 1–2 years were C. pandurata, C. asperata and

  1. rumphii while the seeds can be stored for three years were C. foerstermannii and C. pulverula. Seeds of C.rochussenii and C. celebensis with a short life span should be sown immediately after harvesting, and then preserve them by in vitro culture or by synthetic seeds through encapsulation of protocorm.


Cara Mengutip
Puspitaningtyas DM, Handini E. 2014. PENYIMPANAN BIJI ANGGREK Coelogyne spp. UNTUK KONSERVASI EX SITU. Buletin Kebun Raya 17(2): 101-112. Diambil dari


  1. Anonym. 2014. BD Bionutrients Technical Manual, p:34. nts/Bacto_Peptone.pdf. (Diakses 15 Maret 2014).
  2. Arditti, J. 1967. Factors affecting the germination of orchid seeds. Botanical Review, 33:1–97.
  3. Arditti, J. and A.K.A. Ghani. 2000. Numerical and physical properties of orchid seeds and their biological implications. New Phytologist 145: 367–421.
  4. Butcher, D. and S.A. Marlow. 1989. Asymbiotic germination of epiphytic and terrestrial orchids. In Pritchard, H.W. (ed.). Modern methods in orchid conservation: the role of physiology, ecology and management. p. 17–
  5. Cambridge University Press.
  6. De Pauw, M.A., W.R. Remphrey and C.E. Palmer. 1995. The Cytokinin Preference for in vitro germination and protocorm growth of Cypripedium candidum. Annals of Botany 75: 267–275.
  7. Gravendeel, B. 2000. Reorganising the orchid genus Coelogyne: a phylogenetic classification based on morphology and molecules. Nationaal Herbarium Nederland, Universiteit Leiden Branch.
  8. Handini, E. dan D.M. Puspitaningtyas. 2009. Studi penyimpanan biji anggrek Cymbidium finlaysonianum. Prosiding Konservasi Flora Indonesia dalam Mengatasi Dampak
  9. Pemanasan Global, 14 Juli 2009. UPT Balai Konservasi Tumbuhan Kebun Raya ‘Eka Karya’ Bali – LIPI. p.: 183–190.
  10. Hey, G.L. 1963. The mixed orchid house. The Orchid
  11. Review 71(840):196–198.
  12. Humpreys, J.L. 1960. Help Wanted. The Orchid Review 68(802):141–142.
  13. Kano, K. 1965. Studies on media for orchid seed germination Memoirs of the Faculty of Agriculture, Kagawa University, No.20, 74pp.
  14. Knudson, L. 1946. A new nutrient solution for the germination of orchid seed. American Orchid Society Bulletin 14: 214–217.
  15. Pierik, R.L.M. 1987. In vitro culture of higher plant. Martinus Nyhoff Publisher. Netherlands: Departement of Horticulture Agronomy University.
  16. Pritchard, H.W. 1985 Determination of orchid seed viability using fluorescein diacetate (technical report). Plant, Cell and Environment 8:727– 730.
  17. Puspitaningtyas, D.M. dan A. C. Dwiarum. 2012. Orchid conservation of paraphalaenopsis serpentilingua by in vitro culture. International Conference on Biological Science. Faculty of Biology Gadjah Mada University. September 23rd–24th, 2011. p: 478–486.
  18. Puspitaningtyas, D.M. dan E. Handini. 2011. Uji daya simpan biji anggrek Dendrobium stratiotes Rchb.f. Prosiding Seminar Nasional Konservasi Tumbuhan Tropika: Kondisi Terkini dan Tantangan ke Depan. 7 April 2011. p: 60–
  19. Schwallier R., V. Bhoopalan and S. Blackman. 2011. The influence of seed maturation on desiccation tolerance in Phalaenopsis amabilis hybrids. Scientia Horticulturae 128: 136–140.
  20. Seaton, P. T. dan H. W. Pritchard. 2003. Orchid germplasm collection, storage and exchange. ln: K. W. Dixon, S. P. Kell, R. L. Barrett, dan P. J. Cribb (eds). Orchid conservation. Natural History Publications, Kota Kinabalu, Sabah. P. 227–258.
  21. Seaton, P. T. dan M. M. Ramsay. 2005. Growing orchids from Seed. Royal Botanic Gardens, Kew.
  22. Seaton, P.T., J.P. Kendon, H.W. Pritchard, D.M. Puspitaningtyas, and T.R. Marks. 2013. Orchid Conservation: the next ten years. Lankesteriana 13(1-2): 93–101.
  23. Singh, F. 1981. differential staining of orchid seeds for viability testing. American Orchid Society Bulletin. 50(4):416-418.
  24. Swarts, N. D. dan K. W. Dixon. 2009. Terrestrial orchid conservation in the age of extinction. Annals of Botany 104: 543–556.
  25. Tulecke, W., L.H. Weinstein, A. Rutner, and H.J. Laurencot. 1961. The Biochemical Composition of Coconut water (Coconut Milk) as Related to its Use in Plant Tissue Culture. New York: Plant Research Inc.
  26. Untari, R. dan D.M. Puspitaningtyas. 2006. Pengaruh macam bahan organik dan taraf konsentrasi NAA terhadap pertumbuhan anggrek hitam (Coelogyne pandurata Lindl.) di dalam Kultur In Vitro. Biodiversitas 7(4): 378–382.
  27. Vacin, E. and F. Went. 1949. Some pH changes in nutrient solution. Botanical Gazette 110:605– 613.
  28. Van Waes, J.M. and P.C. Debergh. 1986. Adaption of the Tetrazolium Method for Testing the Seed Viability, and Scanning Electron Microscopy Study of Some Western European Orchids. Physiologia Plantarum 66:435-442.
  29. Vejsadova, H. 2006. Factors Affecting Seed Germination and Seedling Growth of Terrestrial Orchids Cultured in Vitro. Acta Biologica Cracoviensia Series Botanica 48(1): 109-113.
  30. World Conservation Monitoring Centre (WCMC). 1995. Indonesian Threatened Plants. Eksplorasi 2(3):8-9.

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