Paphiopedilum supardii Braem & Loeb is one of Slipper Orchids from Kalimantan, which is included in Appendix I of CITES. It is a critically endangered orchid with restricted habitat endemic to Kalimantan, found growing at altitude of 600-900 m above sea level. In Indonesia, this species is categorized as the priority species for conservation. The aim of this research was to conserve this orchid species through seed storage method in deep freezer (-20 oC) then evaluate the seed germination and growth on some medium composition. Four different media composition were used to test the germination i.e. Knudson C with micro nutrient (KCA), modified Knudson C (KC), modified Vacin & Went (VW) and modified leaf fertilizer (HS). Seed viability test was carried out in 0, 1, 2, 3, 6, 9 and 12 months after storage. The result showed that the seeds of Paphiopedilum supardii were able to germinate in four different germination medium after 12 months of storage. The germination test showed that Knudson C medium with micronutrient (KCA) resulted on the best performance of green protocorm, while the other media tend to produce brown protocorm. Unfortunately, KCA gave the lowest percentage of germination. Furthermore, subculture of protocorm for multiplication and rooting phase was the best in Knudson C medium with addition of both micronutrient and organic materials.
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