In vitro conservation of pummelo (Citrus maxima (Burm.) Merr.) would need shoot as explant, because it makes easier in recovery and uses after conservation. Two experiments were conducted to find out an effective method to induce pummelo shoot directly. Leaf, root and epicotyls from in vitro germination of “Adas Duku” pummelo seeds were used as explants in separate experiments. First experiment was conducted in a completely randomized design, using medium combination of MS + BAP (0; 1; 2 ppm) + NAA (0; 0.5; 1 ppm) as treatments. Second experiment was conducted to study the effect of epicotyl explant position in culture to the shoot emergence. The result showed that leaf, root and epicotyl could produce direct shoot organogenesis. Leaf explant (5.5%) produced 1 shoot/explant in MS + BAP 1 ppm in the dark room. Root explant (60%) produced 1 shoot/explant in MS0 in the light room. Epicotyl explant cultured horizontally (30%) produced 1–2 shoot/explants in MS0 in the light room, while in the dark room it produced etiolated shoot in MS0 and MS + BAP 1 ppm. Epicotyl explant cultured vertically in light condition produced 1–3 shoot/explants (100%).
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