Aeschynanthus radicans var. ‘Monalisa’ also known as lipstick flower, is epiphytic genus of the Gesneriaceae. This plant has high potent as parent for hybridization. Knowledge of flower development in accordance with pollen viability from parent plant is needed to increase the success rate of hybridization. This research aimed to determine the flower development in accordance with its pollen viability of A. radicans var. ‘Monalisa’, The macroscopic observation was done for flower development process. Pollen viability test was observed via in vitro germination and staining test for correlation method analyses. The flower development was took 34–35 days from bud initiation until anthesis, while the period of flower blooming was 12–13 days. The type of pollination syndrome is dychogamous protandrous as the time of pollen maturity is in advance before the stigma receptive. The highest pollen viability in Bewbacker and Kwack (BK) media found at the second days of blooming, which had the value for long filaments and short filaments of 55.7% and 56.7%, respectively. The highest pollen viability from staining test found at the stadium first day of blooming, with the value of long filaments and short filaments are 41.8% and 48.0% respectively with 1% aniline blue; and 29.4% and 27.2% respectively with 1% I2KI. The pollen viability in BK media with 1% aniline blue and 1% I2KI were positively correlated.
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