Recording the existing diversity of an area is necessary to be done before the area is developed into a botanic garden. This study aims to determine the composition, structure and community type of Sampit Botanic Gardens to serve as the basis for determining its land use zoning and collection themes. A vegetation analysis was conducted using plot method that was placed in five locations (each 500 m2). All of the trees, sapling and ground vegetation were enumerated. A total of 40 families, 64 genera and 85 species were found within total plot of 2500 m2. The results shows that there are 30 species of trees, 36 species of sapling, and 43 species of ground vegetation. The highest number of trees is found in plot Lb-C (19 species), but plot Lb-D has the highest diversity and evenness indices. The three plots in dryland are dominated by Imperata cylindrica (Lk- A), Acacia mangium (Lk-B) and Melaleuca leucadendra (Lk-E), while the two plots in the wetlands are dominated by Tetramerista glabra (Lb-C) and Palaquium calophyllum (Lb-D). The highest tree density in plot Lb-C (800 trees/ha), while the highest basal area in Lb-D (24.1 m²/ha). Base on NMDS ordination, the vegetation tends to form three communities, namely: 1) Imperata cylindrica - Dicranopteris linearis, 2) Melaleuca leucadendra - Acacia mangium, and 3) Palaquium calophyllum - Tetramerista glabra. Community of P. calophyllum - T. glabra should be maintained as in situ conservation areas. I. cylindrica - D. linearis community can be usedfor physical buildings, thematic parks, nursery and plants development. The collection zone should be placed at the M. leucadendra - A. mangium community, and in part on the P. calophyllum - T. glabra and I. cylindrica - D. linearis communities. Collection theme for Sampit Botanic Garden suggested is ex situ conservation for heath forest.
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