Protocorms of Cymbidium hartinahianum J.B. Comber & R.E. Nasution did not develop well and the seedling showed slow growth during acclimatization process. Gamma ray irradiation was conducted to obtain mutant adapted to higher temperature. Characterization was conducted to observe the result of irradiation. The treatments includede five irradiation doses, i.e. 0, 5, 10, 15 and 20 Gy. The result showed that there were significant differences on the number of shoots, leaves of shoots and protocorms between 5 Gy and control, and also planlets with 15 Gy irradiation showed significant differences with control for the length of the leaf and number of roots. The planlet treated with 15 Gy has the highest number of leaves with an angle > 45°. Protocorms treated with 15 Gy gamma irradiation produced the best planlet performance and can be used for breeding program.
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