Kartika Ning Tyas
Slamet Susanto
Iswari S. Dewi
Nurul Khumaida


Indonesia has high variety of pummelo [Citrus maxima (Burm.) Merr.], but only several cultivar are cultivated extensively. Others became vurnerable and the germplasm has to be conserved  to prevent their extinction. One of the conservation method is in vitro conservation using a slow  growth technique. Factorial experiment was used in designing the experiment, the first factor was MS medium concentration, i.e. 1/2MS and MS. The second factor was sucrose concentration, i.e. 0; 1; 2; and 3%. The results showed that low concentration of MS medium and sucrose reducing the leaf number and shoot length but increasing the root number and length. Based on inhibition of growth, the most reducing growth was planlet on MS without sucrose.


Cara Mengutip
Tyas KN, Susanto S, Dewi IS, Khumaida N. 2012. KONSERVASI PAMELO {Citrus maxima (Burm.) Merr.} DENGAN PENURUNAN KONSENTRASI MEDIUM DAN SUKROSA. Buletin Kebun Raya 15(2): 102-112. Diambil dari https://publikasikr.lipi.go.id/index.php/buletin/article/view/109


  1. Ara, N., M.K. Bashar, M.K.Uddin, and K.M. Khalequzzaman. 2008. Evaluation of pummelo (Citrus grandis L.) cultivars in northern area of Bangladesh Journal of Agricultural Research 46: 65-75.
  2. Catan?, R., E.M. Mitoi, F. Helepciuc, and I. Holobiuc. 2010. In vitro conservation under slow growth conditions of two rare plant species from Caryophyllaceae family. Electronic Journal of Biology 6: 86-91.
  3. Davies, P.J. 2004. The plant hormones : Their Nature, Occurrence, and Functions. In Davies, P.J. (ed). Plant Hormones Biosynthesis, Signal Transduction, Action! Kluwer Academic Publisher. London. p 1- 35.
  4. Desbrunais, A.B., M. Noirot, and A. Charrier. 1992. Slow growth in vitro conservation of coffee (Coffea spp.). Plant Cell Tissue and Organ Culture 31:105-110.
  5. Dewitte, W. and J. Murray. 2002. Regulation of cell division in plants. In Mcmanus, M.T. and B.E. Veit (eds). Meristematic Tissues in Plant Growth and Development. Sheffield Academic Press. London. p 255- 278.
  6. Gahan, P.B. 2007. Totipotency and the cell cycle. In Jain, S.M. and H. Häggman (eds). Protocols for Micropropagation of Woody Trees and Fruits. Springer. The Netherlands. p 3-14.
  7. Javed, F. and S. Ikram. 2008. Effect of sucrose induced osmotic stress on callus growth and biochemical aspect of two wheat genotypes. Pakistan Journal of Botany 40: 1487-1495.
  8. Marschner, H. 1995. Mineral Nutrition of Higher Plant. Cambridge: Academic Press. 859 p.
  9. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.
  10. Niyomdham, C. 1997. Citrus maxima (Burm.) Merr. In Verheij, E.W.M. and E. Coronel (eds). Plant Resources of South-East Asia 2: Edible Fruits and Nuts. Prosea Foundation. Bogor. hlm 153-157.
  11. Rao, N.K. 2004. Plant genetic resources: Advancing conservation and use through biotechnology. African Journal of Biotechnology 3: 136-145.
  12. Srivastava, L.M. 2002. Plant Growth and Development, Hormon and Environment. Academic Press. London. 772 p.
  13. |
  14. Buletin Kebun Raya Vol. 15 No. 2, Juli 2012
  15. Susanto, S. 2004. Perubahan kualitas buah jeruk besar (Citrus grandis (L.) Osbeck) yang disimpan dan dibiarkan di pohon. Hayati 11: 25-29.
  16. Susanto, S. 2010. Laporan Penelitian Program Insentif Riset Terapan 2010: Perbaikan Potensi Pembentukan Buah Jeruk Pamelo Tanpa Biji untuk Meningkatkan Daya Saing Buah Nasional. Departemen Agronomi dan Hortikultura Fakultas Pertanian IPB. Bogor. (tidak diterbitkan)
  17. Taiz, L. and E. Zeiger. 2002. Plant Physiology. The Benjamin/Cummings Publishing Co. Inc. New York. 672 p.
  18. Thorpe, T., C. Stasolla, E.C. Yeung, G-J de Klerk,
  19. A. Roberts, and E.F. George. 2008. The Components of Plant Tissue Culture Media II : Organic Additions, Osmotic and pH Effects, and Support Systems. In George, E.F., M.A. Hall and G-J. de Klerk (eds). Plant Propagation by Tissue Culture Vol. I The Background. Springer. Dordrecht. p 115–174.
  20. Towill, L.E. 2005. Germplasm preservation. In Trigiano, R.N. and D.J. Gray (eds). Plant Development and Biotechnology. CRC Press. New York. p 277-284.
  21. Whiters, L.A. 1985. Cryopreservation and storage of germplasm. In Dixon, D.A. (ed). Plant Cell Culture. IRL Press. Washington. p 169-190.